Human papilloma virus encodes proteins that inhibit the function of

a. Bcl-2.           b. Myc.                        c. p53.             d. PDGF receptor.      e. Rb.

TLS Online TPP Program

#Question id: 18844

#Unit 13. Methods in Biology

In the extraction of DNA from cell with to remove the high protein content from the cell , before phenol treatment which of the following treatment is done 

TLS Online TPP Program

#Question id: 18845

#Unit 13. Methods in Biology

The action of EDTA in EDTA-lysozyme treatment in bacterial DNA extraction is

TLS Online TPP Program

#Question id: 18846

#Unit 13. Methods in Biology

For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. coli cells in which the target protein fused to an affinity tag has been over-expressed.

TLS Online TPP Program

#Question id: 18847

#Unit 13. Methods in Biology

You know that the protein you want to purify from a natural source forms a multimer with multiple sub-units giving a molecular weight in solution much bigger than visualised denatured on SDS-PAGE. There is only a small amount of the target protein in the total protein sample. Which of the following is an appropriate purification strategy?

TLS Online TPP Program

#Question id: 18848

#Unit 13. Methods in Biology

You find that your protein sample loses activity during storage. What can you do about this?

TLS Online TPP Program

#Question id: 18849

#Unit 13. Methods in Biology

Which of these techniques is often considered a suitable "polishing" step in a protein purification strategy?