Your friends Ben and Jane are contemplating having children and seek your genetic counsel. Ben and Jane are unrelated, but both have younger brothers with the same, extremely rare genetic trait. (In fact, Ben and Jane met at a support group meeting for children and families of children with this rare disorder.) Ben’s parents and Jane’s parents are all unaffected.        

 Assume that the trait is autosomal dominant with 80% penetrance in heterozygotes and 100% penetrance in homozygotes. What is the probability that Ben and Jane’s first child will be affected? 

TLS Online TPP Program

#Question id: 18915

#Unit 13. Methods in Biology

In PBR322, A gene having terminal cut with the PstI, on which selectable marker the colony will be selected 

TLS Online TPP Program

#Question id: 18916

#Unit 13. Methods in Biology

In PBR322, A gene having terminal cut with the SalI and BamHI, on which selectable marker the transformed colony will be selected 

TLS Online TPP Program

#Question id: 18917

#Unit 13. Methods in Biology

Transformation of plasmid in requires competent E.coli cells, this competent cells are formed by chemical and electrical treatment causes cell become more porous, the phenomenon is called 

TLS Online TPP Program

#Question id: 18918

#Unit 13. Methods in Biology

Recognition Sequences for Some Type II Restriction Endonucleases 

Match them correctly

TLS Online TPP Program

#Question id: 18919

#Unit 13. Methods in Biology

What are REP1 and REP2 genes in the plasmid used for?

TLS Online TPP Program

#Question id: 18920

#Unit 13. Methods in Biology

Which selection system is used in a yeast plasmid recombinant?