Various sterilization techniques used in plant tissue culture work;

Sterilization technique	Materials sterilized
A) Dry heat	i) Media, culture vessels, other glass plasticwarc, contaminated cultures
B) Autoclaving	ii) Heat labile compounds like GA3, ABA, zeatin, urea,·enzymes, etc.
C) Surface sterilization	iii) Empty glassware, instruments like scalpels, forceps, needles
D) Liquid (membrane filter of         0.45 μm or smaller pore size)	iv) All plant materials to be cultured

TLS Online TPP Program

#Question id: 15844

#Unit 8. Inheritance Biology

You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts^-1 to ts^-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml.  

If the total size of the phage is 5 x 10⁴ bp and the total genetic map length of the phage is 100 m.u., about how far apart are the mutations in base pairs?

TLS Online TPP Program

#Question id: 15845

#Unit 8. Inheritance Biology

You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts-1 to ts-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml. Find out the relative order of sm, ts-1 and ts-2?

TLS Online TPP Program

#Question id: 15846

#Unit 8. Inheritance Biology

You have isolated two temperature-sensitive mutations in phage l that you suspect may be in the same gene. These phage mutants are called ts-1 and ts-2. Each mutant will form plaques at 35˚C but not at 42˚C. You cross ts-1 to ts-2 phage by coinfecting E. coli at the permissive temperature of 35˚C. When the resulting phage lysate is plated at 35˚C you count 10,000 plaques per ml of phage lysate, but when the same phage lysate is plated at 42˚C, there are only 80 plaques per ml. the relative order is sm→ts-2→ts-1, find out the distances you can calculate in m.u.?

TLS Online TPP Program

#Question id: 29094

#Unit 8. Inheritance Biology

A cross was made between pure wild type flower with white color short pollen, F1 plant was allowed to test cross to produced numerous Offspring in following number;

What is approximate distance between Flower color gene and pollen length gene?

TLS Online TPP Program

#Question id: 29095

#Unit 8. Inheritance Biology

The following table summarizes the result of a cross between two strains of Neurospora having the alleles A and a, respectively. The table shows the different patterns of octad arrangement, and the number of ascus observed of each type;

A) According to the graph first two column are from meiosis with no crossover between locus A and_____I_______ 

B) The pattern for first these two columns represent______II_______

C) The distance between the locus A and the centromere is ___III___ map units

TLS Online TPP Program

#Question id: 29103

#Unit 8. Inheritance Biology

Which of the following is a correct prediction about Anaphase?