Various sterilization techniques used in plant tissue culture work;

Sterilization technique	Materials sterilized
A) Dry heat	i) Media, culture vessels, other glass plasticwarc, contaminated cultures
B) Autoclaving	ii) Heat labile compounds like GA3, ABA, zeatin, urea,·enzymes, etc.
C) Surface sterilization	iii) Empty glassware, instruments like scalpels, forceps, needles
D) Liquid (membrane filter of         0.45 μm or smaller pore size)	iv) All plant materials to be cultured

TLS Online TPP Program

#Question id: 26513

#Unit 3. Fundamental Processes

Eukaryotic nuclei contain RNA polymerase I, RNA polymerase II, and RNA polymerase III can be separated by using

TLS Online TPP Program

#Question id: 26514

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits which are

TLS Online TPP Program

#Question id: 26515

#Unit 3. Fundamental Processes

Which of the two technique can be used to separate Eukaryotic RNA pol     

a. DEAE Column 

b. epitope tagging

c. Density gradient centrifugation 

d. UV-vis

TLS Online TPP Program

#Question id: 26516

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG and myc, so FLAG tagged protein  can be separated by using

TLS Online TPP Program

#Question id: 26517

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell. Alexa- 488-labeled antibody –emit……., Alexa-568-labeled antibody emits ……….respectivel

TLS Online TPP Program

#Question id: 26518

#Unit 3. Fundamental Processes

A significant difference between the transcription of eukaryotic and prokaryotic RNAs is, choose the correct option

a.In bacteria, transcription takes place on a DNA template

b.In bacteria takes place on a chromatin template as a domain 

c.In eukaryotes, transcription takes place on a chromatin template as a domain 

d.In eukaryotes transcription takes place on a DNA template