The following growth regulators (GRs)- auxins, cytokinins, ABA and GA3 are used in plant tissue culture, The common range of concentrations used are as follows:

GROWTH REGULATOR(GRs)                                     CONCENTERATION

A) AUXIN                                                                       i) 0.1-3 mg/L

B) GA3                                                                          ii) 0.2 mg/L

C) CYTOKININ                                                           iii) 0.1-1 mg/L

D) ABA

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#Question id: 18852

#Unit 13. Methods in Biology

What is the starting point for selection of a suitable IEX matrix for purification of a recombinant protein?

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#Question id: 18853

#Unit 13. Methods in Biology

Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?

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#Question id: 18854

#Unit 13. Methods in Biology

What properties of a protein does hydrophobic interaction chromatography exploit for purification?

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#Question id: 18855

#Unit 13. Methods in Biology

to elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate?

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#Question id: 18856

#Unit 13. Methods in Biology

A scientist constructs a recombinant bacterial plasmid that contains a gene conferring resistance to the antibiotic ampicillin (amp). The scientist transforms cultures of E. coli bacteria with the recombinant plasmid, then incubates the cultures overnight on plates containing media without ampicillin (amp-) and with ampicillin (amp+).

Assuming the transformation protocol was successful, which of the following best predicts the bacterial growth pattern expected after the incubation period?

 

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#Question id: 18857

#Unit 13. Methods in Biology

Embryonic lethality that sometimes occurs in knockout models can be avoided by...