During the isolation of somaclonal variants such as cell selection contain positive selection, describe by which method that the wild type cell are killed by selection agent while the variant cells remain alive?

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#Question id: 18952

#Unit 13. Methods in Biology

The system is used to determine the molecular diameter of protein molecules in their native state

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#Question id: 18953

#Unit 13. Methods in Biology

These membranes are made on Teflon base: they have high affinity for proteins, are highly mechanically stable like nylon membranes and can be used for provides high binding capacity for proteins and nucleic acids for western, southern, and northern blotting methods.  

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#Question id: 18954

#Unit 13. Methods in Biology

The above diagram showing which of the following technique

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#Question id: 18955

#Unit 13. Methods in Biology

(i) (RFLPs), (ii) (RAPDs), (iii) (VNTR) DNA, and (iv) (SSRs) are the examples of

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#Question id: 18956

#Unit 13. Methods in Biology

It is possible to calculate the number of clones that must be present in a gene library to give a probability of obtaining a particular DNA sequence. This formula is:

 

where N is the number of recombinants, P is the probability and f is the fraction of the genome in one insert. Thus for the E. coli DNA chromosome of 5 × 10 ⁶ bp andan insert size of 20 kb ( f = 0.004), the number of clones needed ( N ) would be 1 × 10 ³ ,with a probability of P will be

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#Question id: 18957

#Unit 13. Methods in Biology

A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are