5 You are studying regulation of the yeast enzyme glutamine synthetase (GS), which is encoded by the GLN1 gene. You have isolated two mutants, designated gln2– and gln3–, that give decreased GS activity. Mating of either gln2– or gln3– haploids to wild type produces heterozygous diploids that show normal amounts of GS expression. When you cross either a gln2– or gln3– haploid to a gln1– strain the resulting diploids show normal expression of GS.

Describe the cis-acting elements in the GLN1 promoter that are evident from these experiments, giving both their position and as much of their function as you can deduce.

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#Question id: 209

#Unit 1. Molecules and their Interaction Relevant to Biology

TPP is a/an ________ of yeast pyruvate decarboxylase.

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#Question id: 8696

#Unit 9. Diversity of Life Forms

There is some evidence that reptiles called cynodonts may have had whisker-like hairs around their mouths. If true, then hair is a shared ________.

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#Question id: 18742

#Unit 13. Methods in Biology

When inserting a DNA fragment, it is possible to have two orientations. If the orientation is controlled, this cloning is referred to as __________

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#Question id: 3851

#Unit 3. Fundamental Processes

Transcription is initiated at sites called:

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#Question id: 19180

#Unit 13. Methods in Biology

If two successive PCRs are carried out, in which PCR there are chances of having a non-specific product?