TLS Online TPP Program

#Question id: 16134


You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
You determine the DNA sequences of PCR products obtained at STS5 using BACs B, C, D, E, and F as PCR templates. The DNA sequences of PCR products obtained using BACs B and C are identical, but they differ by several nucleotide substitutions from the DNA sequences of PCR products obtained using BACs E and F. Oddly, PCR product obtained using BAC D appears to be a mixture of two sequences – one corresponding to the BAC B/ BAC C sequence and the other corresponding to the BAC E/ BAC F sequence. Briefly account for these findings, and explain how a single PCR assay could amplify two different sequences.

#Unit 13. Methods in Biology
  1. PCR just amplifies RNA to which primers bind. One could imagine that the PCR primers could anneal to both sites and amplify both sites.
  2. PCR just amplifies DNA to which primers bind. One could imagine that the PCR primers could anneal to one site and amplify only one site.
  3. PCR just amplifies DNA to which primers bind, One could imagine that the PCR primers could anneal to both sites and amplify both sites.
  4. PCR just amplifies protein to which primers bind. One could imagine that the PCR primers could anneal to both sites and amplify both sites.
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TLS Online TPP Program

#Question id: 10566

#Unit 6. System Physiology – Plant

What happens if this cell is placed in the beaker containing 0.1 M sucrose, Because the water potential of the sucrose solution (Yw = –0.244) is greater than the water potential of the cell (Yw = –0.732 MPa)

a) Water will move from the cell to the sucrose solution

b) From high to low water potential

c) From low to high water potential

d) Water will move from the sucrose solution to the cell

Which of the following statement is CORRECT?

TLS Online TPP Program

#Question id: 10567

#Unit 6. System Physiology – Plant

Cell A is having solute potential of -14 and Pressure potential of 3. Cell B is having Solute potential of -16 and Pressure potential of 4. The water will travel from

TLS Online TPP Program

#Question id: 10568

#Unit 6. System Physiology – Plant

Turgid cell dropped into sucrose solution , the cell has Yp = 0.488 MPa Ys = –0.732 MPa Yw = –0.244 Mpa , 0.3 M Sucrose solution contain Yp = 0 MPa Ys = –0.732 MPa Yw = –0.732 Mpa , Choose the correct option

TLS Online TPP Program

#Question id: 10569

#Unit 6. System Physiology – Plant

Plants that grow in saline environments, called

TLS Online TPP Program

#Question id: 10570

#Unit 6. System Physiology – Plant

The diffusion coefficient (Ds ) is a characteristic of the substance

a) diffusion in liquid is much faster than diffusion in air

b) larger molecules have smaller diffusion coefficients

c) diffusion in air is much faster than diffusion in a liquid

d) Larger molecules have larger diffusion coefficient

Which of the following combination is correct?

TLS Online TPP Program

#Question id: 10571

#Unit 10. Ecological Principles

Uniform spacing patterns in plants such as the creosote bush are most often associated with