TLS Online TPP Program

#Question id: 16136


You obtain 6 BACs (of known order, as shown below) and 7 STSs (of unknown order) that derive from a region of mouse chromosome 16 whose genomic sequence has not yet been finished.   
 
By PCR (using 20-bp primers at either end of each STS), you test each of the 6 BACs for the presence (+) or absence (-) of each of the 7 STSs. You obtain the following results:
 
Is there a second STS at which you would like to sequence PCR products obtained using BACs as templates? If so, which BACs would you test in this way, and what sequencing results might you predict for each of the BACs tested?

#Unit 13. Methods in Biology
  1. STS1 is present more than once; BACs A, B,  would have the same sequence C, E, F would have the same sequence.
  2. STS5 is present more than once; BACs B, C, D, E, and F would have the same sequence.
  3. STS1 is present more than once; BACs A, B, C would have the same sequence E, F would have the same sequence.
  4. STS2 is present more than once; BACs B, C, D would have the different sequence.
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TLS Online TPP Program

#Question id: 8782

#Unit 9. Diversity of Life Forms

A phylogenetic tree is shown for the three domains of life (Eukarya, Archaea, and Bacteria). The tree branches in two directions from the first point, labeled Common ancestor of all life. One branch leads in the direction of Eukarya and Archaea, and the other branch leads to Bacteria. The branch leading to Eukarya and Archaea divide, one branch leading to each domain. The branch leading to Eukarya divides into four branches. One leads to Euglenozoans, and the other three lead to branching points. The first leads to Forams in one direction, and another branching point in the other that leads to Diatoms and Ciliates. The second branching point leads to Red algae in one direction, and a branching point that leads to green algae and land plants in the other direction. The third branching point leads to amoebas in one direction, and a branching point that leads to fungi and animals in the other direction. Land plants, Fungi and Animals are all highlighted.


Cyanobacteria were once called blue-green algae because they are photosynthetic. According to the phylogeny shown, the cyanobacteria are more closely related to gram-positive bacteria than to ________.

TLS Online TPP Program

#Question id: 8783

#Unit 9. Diversity of Life Forms

Which of the following statements is accurate, at least according to our present knowledge?

TLS Online TPP Program

#Question id: 8787

#Unit 9. Diversity of Life Forms

Which of the following statements is accurate, at least according to our present knowledge?

TLS Online TPP Program

#Question id: 8788

#Unit 9. Diversity of Life Forms

A phylogenetic tree is shown for the three domains of life (Eukarya, Archaea, and Bacteria). The tree branches in two directions from the first point, labeled Common ancestor of all life. One branch leads in the direction of Eukarya and Archaea, and the other branch leads to Bacteria. The branch leading to Eukarya and Archaea divide, one branch leading to each domain. The branch leading to Eukarya divides into four branches. One leads to Euglenozoans, and the other three lead to branching points. The first leads to Forams in one direction, and another branching point in the other that leads to Diatoms and Ciliates. The second branching point leads to Red algae in one direction, and a branching point that leads to green algae and land plants in the other direction. The third branching point leads to amoebas in one direction, and a branching point that leads to fungi and animals in the other direction. Land plants, Fungi and Animals are all highlighted.

Examine the figure. If you were a graduate student trying to develop a thesis project that would resolve polytomies, which of the following groups would you study?

TLS Online TPP Program

#Question id: 8789

#Unit 9. Diversity of Life Forms

If additional DNA sequence evidence shows that yeast genes for synthesizing proteins are more similar to protein-synthesizing genes in bacteria than those in archaea, a modern systematist would ________.

TLS Online TPP Program

#Question id: 9261

#Unit 9. Diversity of Life Forms

Which of the following statements about horizontal gene transfer is accurate?