TLS Online TPP Program

#Question id: 16139


 Many mouse genes are “tissue-specific,” that is, they are present throughout the body but are expressed in only one of the animal’s many tissue types. (Other mouse genes are expressed throughout the body, or in multiple tissues.) Geneticists can study the regulation of a mouse gene by fusing the gene’s promoter region to the LacZ coding sequence and injecting the construct to create a transgenic mouse. Fusion of the mouse amylase promoter to LacZ yielded a Pamylase-LacZ construct.              
Mice heterozygous for the resulting Pamylase-LacZ  transgene displayed the LacZ expression exclusively in the pancreas. Would you expect homozygotes for the transgene to also display LacZ expression in the pancreas?.

#Unit 13. Methods in Biology
  1. Mice homozygous for the transgene to display LacZ expression in the pancreas differ  from  the heterozygous mouse does, homozygote is a result of a cross between two heterozygous mice of the opposite transgenic line.
  2. Mice homozygous for the transgene to display LacZ expression in the pancreas is unlikely to the heterozygous mouse does, homozygote is a result of a cross between two homozygous mice of the same transgenic line.
  3. Mice homozygous for the transgene to display LacZ expression in the pancreas as the heterozygous mouse does, homozygote is a result of a cross between two homozygous mice of the different  transgenic line.
  4. Mice homozygous for the transgene to display LacZ expression in the pancreas as the heterozygous mouse does, homozygote is a result of a cross between two heterozygous mice of the same transgenic line.
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TLS Online TPP Program

#Question id: 13102

#Unit 13. Methods in Biology

 With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.
 

 Why is it that having more repeats leads to a band that is higher in the gel?

TLS Online TPP Program

#Question id: 13103

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.
 
 

Why is it that some people show only one band?

TLS Online TPP Program

#Question id: 13104

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction and load each one into a separate well of an agarose gel, and then run the gel.
 

 
Why is it that some people show two bands?

TLS Online TPP Program

#Question id: 13105

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.
  

Given the data so far, for which of the following statement is correct

TLS Online TPP Program

#Question id: 13106

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.

 
 
Why is it that some people only have one band?

TLS Online TPP Program

#Question id: 13107

#Unit 13. Methods in Biology

With the help of DNA fingerprinting which can be used to determine paternity. There are three babies (Baby A, Baby B and Baby C) in a maternity ward, and three sets of confused and worried parents. (Father and Mother #1 are a couple, as are Father and Mother #2, and Father and Mother #3.) 
You do each PCR reaction on chromosome 15 and load each one into a separate well of an agarose gel, and then run the gel.
 

 
Why is it that some people show two bands?