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TLS Online TPP Program
#Question id: 23151
#Unit 13. Methods in Biology
If the concentration of Ca2+ is high inside the cell and fura-2 dye is used to map the concentration which of the following conditions will prevail?
1. Fluorescence will decrease
2. Fluorescence will increase
3. Emission of light at higher wavelength
4. Emission of light at lower wavelength
Which of the following options are correct?
TLS Online TPP Program
#Question id: 23152
#Unit 13. Methods in Biology
A fluorescent dye SNARF-1 that is sensitive to H+ concentrations can be used to monitor the cytosolic pH of
1. Alkaline cells
2. Acidic cells
3. Dead cells
4. Live cells
Which of the following options are incorrect?
TLS Online TPP Program
#Question id: 23153
#Unit 13. Methods in Biology
SNARF-1is a fluorescent dye used to measure which ion concentration?
TLS Online TPP Program
#Question id: 23154
#Unit 13. Methods in Biology
When the organelles pH is acidic the how the SNARF-1 dye measures this acidity?
TLS Online TPP Program
#Question id: 23155
#Unit 13. Methods in Biology
Match the following ions in column I with their relevant dyes column II.
Column I Column II
1. K+ a. Fura-2
2. Ca2+ b. SNARF-1
3. H+ c. Ion potassium green
Which of the following options is correct?
TLS Online TPP Program
#Question id: 23156
#Unit 13. Methods in Biology
Most widely used method of detecting specific proteins, uses an antibody to which a fluorescent dye has been covalently attached