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TLS Online TPP Program

#Question id: 1530

What are the differences and similarities between the transforming genes of retroviruses and those of DNA tumor viruses?

#Unit 4. Cell Communication and Cell Signaling

  1. The oncoproteins encoded by transducing retroviruses are derived from viral replication, whereas those encoded by DNA viruses are derived from normal cell proteins. Both types of viruses express oncoproteins from integrated genomes.

  2. The oncoproteins encoded by transducing retroviruses are derived from normal cell proteins, whereas those encoded by DNA viruses are required for viral replication. Both types of viruses express oncoproteins from integrated genomes.

  3. There is no difference between the transforming genes of retroviruses and DNA tumor viruses. Both types of viruses express oncoproteins from integrated genomes.

  4. The oncoproteins encoded by transducing retroviruses are derived from normal cell proteins, whereas those encoded by DNA viruses are required for viral replication. There is no similarities in both types of viruses.

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TLS Online TPP Program

#Question id: 17659

#Unit 14. Methods in Biology

During your study of the gene for an enzyme you have isolated an amber mutation in the middle of the gene which produces a truncated form of the enzyme. By placing a +1 frameshift mutation a short distance before the amber mutation and a –1 frameshift mutation a short distance after the amber mutation you create a triple mutant that restores expression of a functional, full-length enzyme. 
a) +1 frameshift puts the amber mutation out of frame so that it is no longer read as a stop.
b) -1 frameshift is inserted after the amber so that no longer read as a stop.
c)  -1 frameshift is inserted after the amber to restore the frame of the end of the protein.
d) +1 frameshift puts the amber mutation out of frame so that it restore the frame of the end of the protein.
Which of the following is the correct prediction about the frame shift mutation?
TLS Online TPP Program

#Question id: 17819

#Unit 14. Methods in Biology

During RNA extraction, the homogenate is first treated with
TLS Online TPP Program

#Question id: 17820

#Unit 14. Methods in Biology

During the RNA extraction guanidinium thiocyanate functions as a strong inhibitor of
TLS Online TPP Program

#Question id: 17997

#Unit 14. Methods in Biology

In RNA isolation, for protein free solution what will be the ratio at  nm
TLS Online TPP Program

#Question id: 17998

#Unit 14. Methods in Biology

Eukaryotic mRNA species are isolated by using
TLS Online TPP Program

#Question id: 18074

#Unit 14. Methods in Biology

Affinity chromatography on oligo(dT)-cellulose columns, is used for eukaryotic mRNA extraction, instead of using oligo(dT)-cellulose we can also use