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TLS Online TPP Program
#Question id: 18954
#Unit 13. Methods in Biology
The above diagram showing which of the following technique
TLS Online TPP Program
#Question id: 18955
#Unit 13. Methods in Biology
(i) (RFLPs), (ii) (RAPDs), (iii) (VNTR) DNA, and (iv) (SSRs) are the examples of
TLS Online TPP Program
#Question id: 18956
#Unit 13. Methods in Biology
It is possible to calculate the number of clones that must be present in a gene library to give a probability of obtaining a particular DNA sequence. This formula is:
where N is the number of recombinants, P is the probability and f is the fraction of the genome in one insert. Thus for the E. coli DNA chromosome of 5 × 10 6 bp andan insert size of 20 kb ( f = 0.004), the number of clones needed ( N ) would be 1 × 10 3 ,with a probability of P will be
TLS Online TPP Program
#Question id: 18957
#Unit 13. Methods in Biology
A DNA fragment of 5000bp needs to be isolated from E.coli (genome size 4x103kb) genomic library. The minimum number of independent recombinant clones required to represent this fragment in genomic library are
TLS Online TPP Program
#Question id: 18958
#Unit 13. Methods in Biology
The number of clones to represent this fragment in genomic library with a probability of 95% are
TLS Online TPP Program
#Question id: 18959
#Unit 13. Methods in Biology
Vectors used for construction of genomic libraries, and their capacity is given match them correctly
| A- λ EMBL series | I - 300kb - 1,400 kb |
| B - Cosmids | II - 45 kb |
| C - BACs and YACs (high capacity vectors) | III - 20kb |