What does the cytoplasmic region of cell surface IgM consists of?

TLS Online TPP Program

#Question id: 23168

#Unit 13. Methods in Biology

What are the advantages of using secondary antibodies in indirect immunofluorescence?

1.  It makes easy to choose a different conjugate no matter the primary antibody

2. Enhance detection by localizing more conjugate at the antigen

3. Conjugation of fluorophore can interfere with the primary antibody’s recognition of the antigen

Which of the following options is correct?

TLS Online TPP Program

#Question id: 23169

#Unit 13. Methods in Biology

Indirect immunofluorescence microscopy uses an unlabeled primary antibody, followed by a fluorescently labeled 

TLS Online TPP Program

#Question id: 23170

#Unit 13. Methods in Biology

Which epitope tags can be used to tag recombinant proteins?

1. FLAG

2. myc

3. TICK

Which of the following options is incorrect?

TLS Online TPP Program

#Question id: 23171

#Unit 13. Methods in Biology

Using fluorescently labeled epitope tags in microscopy actually give advantage to visualize

TLS Online TPP Program

#Question id: 23172

#Unit 13. Methods in Biology

Green fluorescent protein do contains a tripeptide sequence i.e. ser-tyr-gly but it only fluoresce when 

TLS Online TPP Program

#Question id: 23173

#Unit 13. Methods in Biology

There are various drawbacks still in fluorescence microscopy such as

1.  Light emitted comes from molecules above and below the plane of focus

2. Blurred image due to superposition of fluorescent images

Which of the above statement is/ are correct?