#Question id: 5279
#Unit 8. Inheritance Biology
In a transduction experiment, phage P1 is grown on a bacterial host of genotype A+ B+ C+ and the resulting lysate is used to infect a recipient strain of genotype A– B– C–. The genes are in an order such that B is in the middle, and the distance between A and B is greater than the distance between B and C. Based on this information, which of the following least genotype?
#Question id: 5280
#Unit 8. Inheritance Biology
In the mating Hfr met- his+ leu+ trp+ × F- met+ his- leu- trp-. The met marker is known to be transferred very late. After a short time, the mating cells are interrupted and the cell suspensions plated on four different growth media. The amino acids in the growth media and the number of colonies observed on each are as follows:
histidine + tryptophan 250 colonies
histidine + leucine 50 colonies
leucine + tryptophan 500 colonies
histidine 10 colonies
What is the order of transfer of the genes
#Question id: 5281
#Unit 8. Inheritance Biology
In the mating Hfr Strs met+ his+ leu+ trp+ Ala+ X F- Strres met- his- leu- trp- Ala-. At regular interval time, the mating cells are interrupted and the cell suspensions plated on four different growth media with streptomycin. The amino acids in the growth media and the number of colonies observed on each are as follows:
|
Interrupted time |
Composition of media |
Growth |
||||
|
His |
Leu |
Ala |
Trp |
Met |
||
|
2 min |
+ |
- |
+ |
+ |
+ |
Yes |
|
10 min |
+ |
- |
- |
+ |
+ |
Yes |
|
12 min |
+ |
- |
- |
+ |
- |
No |
|
15 min |
+ |
- |
- |
- |
+ |
Yes |
|
20 min |
- |
- |
- |
- |
+ |
Yes |
What is the order of transfer of the genes?
#Question id: 5282
#Unit 8. Inheritance Biology
Conjugation allowed between Hfr with genotype A B C D E and F- with genotype a b c d e. At regular intervals, a sample of cells was removed and agitated
vigorously in a kitchen blender to halt all conjugation and DNA transfer. After interrupted conjugation, ex-conjugate were selected on minimal media at regular interval and find different number of ex-conjugate?
abCde - 10, abCdE - 30 , aBCdE - 40 , ABCdE - 20 , ABCDE - 50
What is gene order of those genes?
#Question id: 5283
#Unit 8. Inheritance Biology
A generalized transducing phage is used to transduce an a- b- c- d- e- recipient strain of E. coli with an a+ b+c+d+ e+ donor. The recipient culture is plated on various media with the results shown in the table below What can you conclude about the linkage and order of the genes?
#Question id: 5284
#Unit 8. Inheritance Biology
DNA from a strain of Bacillus subtilis with genotype a+b+ c+d+ e+ is used to transform a strain with genotype a - b- c- d- e-. Pairs of genes are checked for co-transformation and the following results are obtained:
Pair of co-transform genes as follow
D-E, E-C, C-B, A-D
On the basis of these results, what is the order of the genes on the bacterial chromosome?