Variants of PCR has been devised to enhance efficiency are

a) qPCR with SYBR, TaqMan and MB

b) nested PCR

c) Touchdown start PCR

d) hot start PCR

TLS Online TPP Program

#Question id: 26514

#Unit 3. Fundamental Processes

The α-amanitin binding site is beneath a “bridge helix” extending across the cleft between the two largest pol II subunits which are

TLS Online TPP Program

#Question id: 26515

#Unit 3. Fundamental Processes

Which of the two technique can be used to separate Eukaryotic RNA pol     

a. DEAE Column 

b. epitope tagging

c. Density gradient centrifugation 

d. UV-vis

TLS Online TPP Program

#Question id: 26516

#Unit 3. Fundamental Processes

Two commonly used epitope tags are FLAG and myc, so FLAG tagged protein  can be separated by using

TLS Online TPP Program

#Question id: 26517

#Unit 3. Fundamental Processes

Fluorochrome-coupled monoclonal antibodies to the FLAG or myc epitopes can then be used to detect the recombinant protein in the cell. Alexa- 488-labeled antibody –emit……., Alexa-568-labeled antibody emits ……….respectivel

TLS Online TPP Program

#Question id: 26518

#Unit 3. Fundamental Processes

A significant difference between the transcription of eukaryotic and prokaryotic RNAs is, choose the correct option

a.In bacteria, transcription takes place on a DNA template

b.In bacteria takes place on a chromatin template as a domain 

c.In eukaryotes, transcription takes place on a chromatin template as a domain 

d.In eukaryotes transcription takes place on a DNA template

TLS Online TPP Program

#Question id: 26519

#Unit 3. Fundamental Processes

A regulatory site that binds more negative regulators than positive regulators to control transcription is called a