TLS Online TPP Program

#Question id: 18702


What would be the effect on the PCR reaction if any of the following circumstances arose: 1) there are no primers in the reaction, 2) there are no dNTPs in the reaction, 3) there is no Taq polymerase in the reaction?

#Unit 13. Methods in Biology
  1. PCR would proceed normally
  2. Non-specific PCR of random templates will occur
  3. The reaction will cease after a few cycles
  4. The PCR reaction will not commence
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TLS Online TPP Program

#Question id: 4547

#Unit 3. Fundamental Processes

Transcription always done in 

TLS Online TPP Program

#Question id: 4548

#Unit 3. Fundamental Processes

 σ- subunit of prokaryotic RNA polymerase describing is incorrect

TLS Online TPP Program

#Question id: 4549

#Unit 3. Fundamental Processes

Which of the following is the correct combination?                             

I) A- Holoenzyme, B- Core

II) A- Core, B- Holoenzyme

i. The six subunits constitute the RNA polymerase an enzyme

ii. Sigma stimulates tight binding between RNA polymerase and promote

iii. absence of sigma factor polymerase loosely bind with their promoter

Which of the following combination correct fit by follow the given graph;

TLS Online TPP Program

#Question id: 4550

#Unit 3. Fundamental Processes

Which one of the following factor of Holoenzyme assists the RNA polymerase in separating the DNA strands at the transcription start site?

TLS Online TPP Program

#Question id: 4551

#Unit 3. Fundamental Processes

Various types of σ factor in column I, that are involved in several other function given in column II

 I

II

I) σ70

a) Induced by unfolded proteins in the cytoplasm contain chaperones activity which refold unfolded proteins               

II) σs

b) Genes involved in flagellum assembly             

III) σ32

c) Genes for nitrogen metabolism and other functions

IV) σE

d) Housekeeping genes, most genes in exponentially replicating cells

V) σF

e) Activated by unfolded proteins in the periplasmic space and cell membrane; genes encoding proteins that restore integrity to the cellular envelope

VI) σ54

f) Stationary-phase genes and general stress response

 Which of the following combination is correct?

TLS Online TPP Program

#Question id: 4552

#Unit 3. Fundamental Processes

The sequence of σ54 is distinctly different from that of all the σ70-like factors, some characteristics about σ54 is given below;

a) The σ54-RNA polymerase binds to the glnA promoter and immediately melt the DNA strands and initiate transcription

b) σ54-polymerase does not require ATP hydrolysis to separate the strands at a start site only require activator such as   NtrC

c) σ54-RNA polymerase initiate transcription of glnA promoter only when it is activated by NtrC

d) ATP hydrolysis is required for activation of bound σ54-RNA polymerase by phosphorylated NtrC

Which of the following from the bove combination is INCORRECT?