TLS Online TPP Program

#Question id: 18803


Carboxyfluorescein succinimidyl ester (CFSE)is non-toxic to cells hence retained till many cycles in CFSE labeling amount of fluorescence emitted is cut in half each time the cell divides, after labelling the following graph is obtained 
 
In this graph 

#Unit 13. Methods in Biology
  1. all the peaks represents the undivided cell
  2. both the peak represents the undivided cell 
  3. The peak to its immediate left represents cells that is undivided even once, the next one to the left represents cells that have divided once
  4. The peak to its immediate left represents cells that have divided once, the next one to the left represents cells that have divided twice
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TLS Online TPP Program

#Question id: 4063

#Unit 3. Fundamental Processes

Which statement correctly matches the three RNA polymerases with RNAs they synthesize?

TLS Online TPP Program

#Question id: 4064

#Unit 3. Fundamental Processes

Which of the following mutations can result in a reduction of β-galactosidase?

TLS Online TPP Program

#Question id: 4065

#Unit 3. Fundamental Processes

The NtrC protein from E. coli

a. is a σ54 RNA polymerase.  b. stimulates transcription of the glnA gene.

c. is activated by a protein kinase called NtrB.

d. binds both to an enhancer site upstream of the glnA gene and to RNA polymerase.

e. has ATPase activity.

TLS Online TPP Program

#Question id: 4066

#Unit 3. Fundamental Processes

Specific DNA control elements in promoters can

a. interact with general transcription factors.

b. interact with repressor proteins. 

c. interact with activator proteins.

d. remain unavailable because of condensed chromatin.

TLS Online TPP Program

#Question id: 4067

#Unit 3. Fundamental Processes

Reporter genes are used to

a. express enzymes that are not easily assayed in cell extracts.

b. express enzymes that are easily assayed in cell extracts.

c. characterize DNA control elements. 

d. characterize reporter plasmids.

TLS Online TPP Program

#Question id: 4068

#Unit 3. Fundamental Processes

The three eukaryotic RNA polymerases can be distinguished by

a. the types of genes they transcribe.

b. the number and types of large subunits.

c. their differential sensitivities to cycloheximide.

d. their differential sensitivities to α-amanitin.