TLS Online TPP Program

#Question id: 18835


Polymerase chain reaction (PCR) utilizes a heat-stable polymerase (Taq polymerase) to amplify DNA strand. Which of the statement best describes why thermo-stable polymerases are required for PCR?

#Unit 13. Methods in Biology
  1. PCR needs thermal cycling, and the Taq polymerases can be inactivated since they are not needed during the low-temperature steps. 
  2. Heat stable (Taq) polymerase do not break the dsDNA unless the temperature is very high. Therefore a heat-stable (Taq) polymerase is required.
  3. PCR needs thermal cycling, and the heat-stable (Taq) polymerases will not denature to lose efficacy in DNA polymerization during the high temperature (95oC) step. 
  4. The interaction between divalent cations and DNA polymerase is much efficient during high-temperature steps. Thus PCR requires heat-stable DNA polymerase.
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TLS Online TPP Program

#Question id: 10897

#Unit 10. Ecological Principles

When three species of sunfish with similar niches were all placed in the same experimental pond, each made use of resources that they did not exploit in the absence of the other species. This scenario is referred to as

TLS Online TPP Program

#Question id: 10898

#Unit 10. Ecological Principles

Living in groups, such as the herds of bison that once inhabited the prairie, may evolve because of

TLS Online TPP Program

#Question id: 10899

#Unit 6. System Physiology – Plant

Companion cells transport of photosynthetic products from producing cells in mature leaves to the sieve elements in the minor veins of the leaf. There are at least three different types of companion cells in the minor veins of mature, exporting leaves, All three cell types have dense cytoplasm and abundant mitochondria;

TLS Online TPP Program

#Question id: 10900

#Unit 6. System Physiology – Plant

companion cells in minor veins of mature leaves. All three cell types have dense cytoplasm and abundant mitochondria, there are three types companion cells  present in column A while its characteristics given in column B;

             COLUMN A                                                               COLUMN B

A) Ordinary companion cells             i) appear well suited for taking up solutes via cytoplasmic connections numerous plasmodesmata connecting them to bundle sheath cells. Its having numerous small vacuoles, as well as poorly developed thylakoids.

 

B) Transfer cells                                 ii) have chloroplasts with well-developed thylakoids     and a cell wall with a smooth inner surface. The number of plasmodesmata connecting ordinary companion cells to surrounding cells.

 C) Intermediary cells                          iii) the development of fingerlike wall in growths, particularly on the cell Walls. These wall ingrowths greatly increase the surface area of the transfer cell’s  plasma membrane, few plasmodesmata connect this type of companion cell to any of the surrounding cells except its own sieve element.

Which of the following correct combination;

TLS Online TPP Program

#Question id: 10901

#Unit 6. System Physiology – Plant

P-proteins have been characterized at the molecular level. P-proteins from the genus Cucurbita consist of two major proteins;

TLS Online TPP Program

#Question id: 10902

#Unit 6. System Physiology – Plant

P-proteins from the genus Cucurbita consist of two major proteins: PP1, the phloem filament protein, and PP2, the phloem lectin. Both PP1 and PP2 are thought to be synthesized in_______A___________and transported via the_______B_________to the_______C______, where they associate to form P-protein filaments and P-protein bodies.