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TLS Online TPP Program

#Question id: 18835

Polymerase chain reaction (PCR) utilizes a heat-stable polymerase (Taq polymerase) to amplify DNA strand. Which of the statement best describes why thermo-stable polymerases are required for PCR?

#Unit 13. Methods in Biology
  1. PCR needs thermal cycling, and the Taq polymerases can be inactivated since they are not needed during the low-temperature steps. 
  2. Heat stable (Taq) polymerase do not break the dsDNA unless the temperature is very high. Therefore a heat-stable (Taq) polymerase is required.
  3. PCR needs thermal cycling, and the heat-stable (Taq) polymerases will not denature to lose efficacy in DNA polymerization during the high temperature (95oC) step. 
  4. The interaction between divalent cations and DNA polymerase is much efficient during high-temperature steps. Thus PCR requires heat-stable DNA polymerase.
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TLS Online TPP Program

#Question id: 14839

#Unit 5. Developmental Biology

Given are certain facts which define ‘specification’ of a developing embryo.
 A. At the specification stage, commitment to cell identity is labile.
 B. At the specification stage, cell is capable of differentiating autonomously.
 C. The cell “knows” very early what it is to become without interacting with other cells.
 D. A phase where inductive signals trigger cell to determines their fate.
 Which of the above statements best define specification?
TLS Online TPP Program

#Question id: 14840

#Unit 5. Developmental Biology

If a cell or tissue type is able to differentiate according to its specified fate, it is assumed that commitment is:
TLS Online TPP Program

#Question id: 14841

#Unit 5. Developmental Biology

How cells became different from one another during embryonic development?
TLS Online TPP Program

#Question id: 14842

#Unit 5. Developmental Biology

If a specified cell is transplanted to a population of differently specified cells, the fate of the transplant will be:
A. Altered if the specification is reversible
B. Capable of differentiating by itself if the specification is reversible
C. Altered if the specification is irreversible
D. Capable of differentiating by itself if the specification is irreversible
Which of the following combination is correct?
TLS Online TPP Program

#Question id: 14843

#Unit 5. Developmental Biology

In both animals and plants, undifferentiated cells go through a process of maturation that begins when they become committed to a specific cell lineage.
   
Which of the following option represents correct events regarding to series

TLS Online TPP Program

#Question id: 15245

#Unit 5. Developmental Biology

which of the following is property of Syncytial specification?