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TLS Online TPP Program

#Question id: 18846

For an application where you require a sample of your target protein at high purity, what would be a good purification strategy? Assume that your starting point is E. coli cells in which the target protein fused to an affinity tag has been over-expressed.

#Unit 13. Methods in Biology
  1. Affinity chromatography (AC) followed by size exclusion chromatography (SEC)
  2. AC only
  3. AC followed by ion-exchange (IEX) followed by SEC
  4. AC followed by IEX, followed by hydrophobic interaction (HIC) and then SEC
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TLS Online TPP Program

#Question id: 15751

#Unit 12. Applied Biology

Plants through the fusion of protoplasts of two different plant species/varieties is called
TLS Online TPP Program

#Question id: 28253

#Unit 5. Developmental Biology

 In invertebrates, an extracellular matrix that forms a fibrous mat around the egg and is involved in sperm-egg recognition is called as____
TLS Online TPP Program

#Question id: 28315

#Unit 5. Developmental Biology

Which of the following protein that digests zona and responsible for digesting a pathway to the oocyte?
TLS Online TPP Program

#Question id: 19792

#Unit 12. Applied Biology

Expression of BtR175 gene in mammalian cells enables Cry1Aa to bind the cells and cause their lysis. The specificity seems to be lost upon reduction of which amino acid residues of the protoxin, but can be restored by reoxidation of these residues?
TLS Online TPP Program

#Question id: 12396

#Unit 6. System Physiology – Plant

Which compound  are included in the buffer washout solution, that involved in prevent normal relocalization of PIN to the plasma membrane ?