You know that the protein you want to purify from a natural source forms a multimer with multiple sub-units giving a molecular weight in solution much bigger than visualised denatured on SDS-PAGE. There is only a small amount of the target protein in the total protein sample. Which of the following is an appropriate purification strategy?

TLS Online TPP Program

#Question id: 1228

#Unit 4. Cell Communication and Cell Signaling

Place the events that follow the binding of a ligand to a G protein-coupled receptor that acts on adenylyl cyclase in the correct order:

a. GTP is hydrolyzed to GDP.           

b. GDP is displaced from Gα.           

c. the Gα-GTP complex dissociates from the Gβγcomplex.  

d. GTP is bound to Gα. 

TLS Online TPP Program

#Question id: 4655

#Unit 11. Evolution and Behavior

Over evolutionary time, many cave-dwelling organisms have lost their eyes. Tapeworms have lost their digestive systems. Whales have lost their hind limbs. How can natural selection account for these losses?

TLS Online TPP Program

#Question id: 19230

#Unit 12. Applied Biology

A physical gene transformation method that can be used with a wide range of plants and tissues; easy and inexpensive

TLS Online TPP Program

#Question id: 14757

#Unit 5. Developmental Biology

Which of the following events are responsible for transition from fertilization to cleavage ?

TLS Online TPP Program

#Question id: 11853

#General Aptitude

Find the least number which when divided by 6,7,8,9 and 12 leaves the same remainder 1 in each case.