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#Question id: 18853


Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?

#Unit 13. Methods in Biology
  1. Measure a UV absorbance scan and use the absorbance at 280nm with the molar extinction coefficient (predicted from the amino acid sequence)
  2. Determine amino acid composition after hydrolysis to amino acids
  3. Colorimetric assay using Bradford or BCA assays
  4. "Guesstimate" the concentration from the intensity of bands on SDS-PAGE gel
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TLS Online TPP Program

#Question id: 9577

#Unit 10. Ecological Principles

The epidermal coloration of the African grasshopper is a striking example of irreversible developmental responses to environmental conditions. Which of the following statements is true?

TLS Online TPP Program

#Question id: 9578

#Unit 10. Ecological Principles

Bergmann’s rule states that,

TLS Online TPP Program

#Question id: 9579

#Unit 10. Ecological Principles

Allen’s rule states that,

TLS Online TPP Program

#Question id: 9580

#Unit 6. System Physiology – Plant

Many Antenna Complexes Have a common structural motif In all eukaryotic photosynthetic organisms that contain both chlorophyll a and chlorophyll b, the most abundant antenna proteins are members of a large family of structurally related proteins, some of these proteins are associated with PSI and PSII such as LHCI and LHCII , Select the incorrect one regarding LHC complexes;

TLS Online TPP Program

#Question id: 9581

#Unit 6. System Physiology – Plant

These four Photosystem II, Cytochrome b6 f, Photosystem I and ATP synthase integral membrane complexes are vectorially oriented in the thylakoid membrane to function as follows, find out the incorrect statements;

TLS Online TPP Program

#Question id: 9582

#Unit 6. System Physiology – Plant

Electrons ejected from chlorophyll travel through a series of electron carriers organized in the “Z Scheme” shows a current version of the Z scheme, in which all the electron carriers known to function in electron flow from H2O to NADP Presented in the following sequence;

a.) P680 reduce by Yz has received electrons from oxidation of water P680→P680*→pheophytinplastoquinones→ cytochrome b6 f complex→ plastocyanin→ in turn reduces (PSI) P700+

b.) P700+→ P700*→ membrane-bound iron–sulfur proteins → A0→ A1 → flavoprotein ferredoxin–NADP reductase (FNR) soluble → ferredoxin(Fd) → NADP+ to NADPH

c.) P700+→ P700*→ A0→ A1→ membrane-bound iron–sulfur proteins→ ferredoxin(Fd)→ soluble flavoprotein ferredoxin–NADP reductase (FNR)→ NADP+ to NADPH

d.) P680 reduce by Yz has received electrons from oxidation of water P680→P680*→ plastoquinonespheophytin → cytochrome b6 f complex→ plastocyanin→ in turn reduces (PSI) P700+

Which one of the following combinations is correct?