Your purification strategy of combinations of chromatography steps gives a protein preparation with a single band on SDS-PAGE. Which of the following would be best for determining the protein concentration (as mg/ml or molarity)?

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#Question id: 23110

#Unit 2. Cellular Organization

Individual nucleosomes can be obtained by treating chromatin with the endonuclease micrococcal nuclease (MNase), which cuts the DNA between nucleosomes, a region known as 

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#Question id: 23111

#Unit 2. Cellular Organization

Choose the correct option 

a. Individual nucleosomes can be obtained by treating chromatin with the endonuclease micrococcal nuclease (MNase), which cuts the DNA between nucleosomes, a region known as H2A-H2B dimer region. 

b. Individual nucleosomes can be obtained by treating chromatin with the endonuclease micrococcal nuclease (MNase), which cuts the DNA between nucleosomes, a region known as linker DNA.

c. Mononucleosomes typically have 200 bp DNA. End-trimming reduces the length of DNA first to 165 bp and then generates core particles with 146 bp.

d. Mononucleosomes typically have 200 bp DNA. End-trimming reduces the length of DNA first to 165 bp and then generates core particles with 200 bp.

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#Question id: 23112

#Unit 2. Cellular Organization

Chromatin on treatment with Mnase generates fragments, as the time and concentration of Mnase increase various fragments are observed choose the incorrect statement 

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#Question id: 23113

#Unit 2. Cellular Organization

Chromatin on digestion with Mnase can be result on centrifugation and electrophoresis, on sedimentation the following result is obtained 

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#Question id: 23114

#Unit 2. Cellular Organization

What is the minimum length to stabilize nucleosome 

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#Question id: 23115

#Unit 2. Cellular Organization

The assay which is used in DNA foot-printing to identify the nitrogenous base sequences