TLS Online TPP Program

#Question id: 18855


to elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate?

#Unit 13. Methods in Biology
  1. Low salt concentrations
  2. High salt concentrations
  3. Adding a soluble ligand which competes with the affinity tagged protein for binding to the column
  4. Just keep washing buffer through the column, isocratic elution