TLS Online TPP Program

#Question id: 18855


to elute target proteins from an affinity chromatography matrix, which of the following conditions would be the most appropriate?

#Unit 13. Methods in Biology
  1. Low salt concentrations
  2. High salt concentrations
  3. Adding a soluble ligand which competes with the affinity tagged protein for binding to the column
  4. Just keep washing buffer through the column, isocratic elution
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TLS Online TPP Program

#Question id: 28853

#Unit 2. Cellular Organization

Stathmin enhances that a growing microtubule will switch to the shrinking state in which condition?
a) When it increases the effective concentration of tubulin subunits that are available for polymerization 
b) When it decreases the effective concentration of tubulin subunits that are available for polymerization 
c) When stathmin is in phosphorylated condition
d) When stathmin is in dephosphorylated condition

TLS Online TPP Program

#Question id: 28854

#Unit 2. Cellular Organization

which protein promotes breakage by extracting tubulin subunits from the wall of the microtubule (also releases microtubules from MTOCs) ?

TLS Online TPP Program

#Question id: 28855

#Unit 2. Cellular Organization

which protein promotes breakage by extracting tubulin subunits from the wall of the microtubule (also releases microtubules from MTOCs) ?

TLS Online TPP Program

#Question id: 28856

#Unit 2. Cellular Organization

The main families of motor proteins mediate transport along microtubules Kinesin-1 which is similar to myosin II that contain various domains such as
I) Head domain
II) Stalk domain
III) Tail domain
a) forms a long coiled-coil
b) at the C-terminus that attaches to cargo
c) at the N-terminus of the heavy chain
d) at the C-terminus of the heavy chain

Correctly match the following domains with given characters;

TLS Online TPP Program

#Question id: 28857

#Unit 2. Cellular Organization

Which of the following motor proteins mediate the  transport along microtubules similar to myosin II ?

TLS Online TPP Program

#Question id: 28858

#Unit 2. Cellular Organization

Which of the following kinesin is a C-terminal kinesin & travel toward the microtubule minus end?