TLS Online TPP Program

#Question id: 18927


Which of the following statements, if any, is false?

#Unit 13. Methods in Biology
  1. The polymerase chain reaction (PCR) is a cell-free method of DNA amplification.
  2. PCR is usually used to amplify a specific DNA sequence of interest using oligonucleotide primers that bind to closely flanking sequences.
  3. PCR is superior to cell-based DNA cloning for two major reasons: it is much quicker and it allows much greater DNA amplification.
  4. PCR requires the use of a heat-stable DNA polymerase to make copies of the template DNA.
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TLS Online TPP Program

#Question id: 4503

#Unit 3. Fundamental Processes

Pol I transcribes the human rRNA genes, the promoter for the rRNA gene comprises two parts: the core element and the UCE (upstream control element), for initiation there are two factors-

TLS Online TPP Program

#Question id: 4504

#Unit 3. Fundamental Processes

What will be the molecular weight of poly(A) chain consisting of 100 residues, where weight of AMP is 500?

TLS Online TPP Program

#Question id: 4505

#Unit 3. Fundamental Processes

Symplekin protein is participats in which of the following processes;

TLS Online TPP Program

#Question id: 4506

#Unit 3. Fundamental Processes

Which process is very much similar to polyadenylation, because its takes place by the multiple protein complexes,

TLS Online TPP Program

#Question id: 4507

#Unit 3. Fundamental Processes

A group of introns that-unlike those we have considered thus far-can splice themselves out of pre-mRNA without the need for the spliceosome, they are called

TLS Online TPP Program

#Question id: 4508

#Unit 3. Fundamental Processes

Statement: In the case of group II introns, the chemistry of splicing and the RNA intermediates produced are the same as those for nuclear pre-mRNA.

 Explanations: I. The intron uses an A residue within the branch site to attack the phosphodiester bond at the boundary between its 5’ end and the end of the 5’ exon-that is, at the 5’ splice site. This reaction produces the branched lariat.

II. A second reaction in which the newly freed 3’ -OH of the exon attacks the 3’splice site, releasing the intron as a lariat and fusing the 3’ and 5’ exons.