TLS Online TPP Program

#Question id: 18928


With respect to nucleic acid hybridization, which, if any, of the following statements is false?

#Unit 13. Methods in Biology
  1. The probe is a labeled nucleic acid or oligonucleotide that is expected to hybridize to a target DNA sequence within an unlabelled test nucleic acid population.
  2. The probe is a known single-stranded nucleic acid or oligonucleotide that is intended to hybridize to complementary target sequences in a poorly understood nucleic acid test sample.
  3. The object of a hybridization assay is to identify target sequences within a test sample that are related to the probe so that some new information is gained about the target sequences.
  4. During a hybridization assay, a heteroduplex is formed by un-natural base pairing between complementary probe and target sequences that show a sufficiently high degree of base pairing across part or all of their lengths.