TLS Online TPP Program

#Question id: 18941


With the construction of cDNA libraries in which each gene is fused to an epitope tag, investigators can precipitate the protein product of a gene by complexing it with the antibody that binds the epitope, a process called 

#Unit 13. Methods in Biology
  1. immunoprecipitation
  2. pulsed field gel electrophoresis
  3. site-directed mutagenesis
  4. polymerase chain reaction (PCR)
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TLS Online TPP Program

#Question id: 11748

#Unit 6. System Physiology – Plant

In mature cells, P-protein occurs in several different forms depending on the species. Such as;

TLS Online TPP Program

#Question id: 11802

#Unit 6. System Physiology – Plant

Damaged sieve elements are sealed off, but because of the processs that plug sieve plates pores, excuded phloem sap from wounds that sever sieve elements. Exudation of sap enhanced by the inclusion of;

TLS Online TPP Program

#Question id: 11803

#Unit 6. System Physiology – Plant

Due to damage exudation of sap from cut petioles or stems takes place they can be collected. However, quantities of collected sap are small, and the method is technically difficult. A preferable approach for collecting exuded sap is to use an

TLS Online TPP Program

#Question id: 11807

#Unit 6. System Physiology – Plant

Given below are few statements regarding the cytokinin signalling pathway;

a) Cytokinin inhibits the activation of a Cdc25- like phosphatase, whose role is to remove an inhibitory phosphate group from the Cdc2 kinase

b) Cytokinin oxidase irreversibly inactivates cytokinins, and it could be important in regulating or limiting cytokinin effects

c) Cytokinin signal transduction pathway is completed on multiple places such as ER, Cytosol and Nucleus

d) Elevation of endogenous cytokinin levels in transgenic Arabidopsis results in overexpression of the KNOTTED homeobox transcription factor homologs KNAT1 and STM—genes 

Which of the following combinations  from the given above statements is correct?

TLS Online TPP Program

#Question id: 11808

#Unit 6. System Physiology – Plant

In addition to the free bases, which are the only active forms, active cytokinin levels are decreased through;

a) Addition of sugar molecule to the 3, 7, or 9 nitrogen of the purine ring, called glycosylation-mediated inactivation

b) cis-trans isomerisation of zeatin

c) Irreversible cleavage by cytokinin oxidases.

Which of the following is incorrect?

TLS Online TPP Program

#Question id: 11809

#Unit 6. System Physiology – Plant

Shortly after the discovery of kinetin, it was observed that the differentiation of cultured callus tissue derived from tobacco pith segments into either roots or shoots depends on the ratio of auxin to cytokinin in the culture medium. Whereas

a) high auxin:cytokinin ratios stimulated the formation of roots

b) high auxin:cytokinin ratios stimulated the formation of shoots

c) low auxin:cytokinin ratios led to the formation of shoots

d) low auxin:cytokinin ratios stimulated the formation of roots

which of the following combination is correct about the ratio of auxin to cytokinin