The use of wild type pTi as a vector presents the following three problems.

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.

2. Their large size makes the handling procedures during cloning tedious and cumbersome.

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned

In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop

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#Question id: 27929

#Unit 11. Evolution and Behavior

Which of the following phenomenon could be used to directly examine genetic variation?

a) RPLC

b) RFLP

c) DNA sequencing

d) NPLC

TLS Online TPP Program

#Question id: 1708

#Unit 4. Cell Communication and Cell Signaling

When antigen reaches a lymph node in a primed animal:

TLS Online TPP Program

#Question id: 14501

#Unit 13. Methods in Biology

In a population having 50% rice consumers, what is the probability that three or less out of 10 are rice consumers?

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#Question id: 5289

#Unit 8. Inheritance Biology

A donor strain of bacteria with genes a+b+c+ is infected with phages to map the donor chromosome with transduction. The phage lysate from the bacterial cells is collected and used to infect a second strain of bacteria that are a_ b_ c_.

Based on the above, which one of the following conclusions is correct?

TLS Online TPP Program

#Question id: 28073

#Unit 6. System Physiology – Plant

Which of the following is correct regarding ATP & NADPH synthesis and its physiological environment 

(a) ATP & NADPH is synthesized in Stroma

(b) ATP & NADPH is synthesized in Lumen

(c) STROMA is alkaline and similar to Mitochondrial matrix

(d) STROMA is acidic and similar to Mitochondrial matrix

Which of the following is correct