The use of wild type pTi as a vector presents the following three problems.

1. Presence of oncogenes (iaaM, iaaH and ipt) in T-DNA, which causes a disorganised growth and a loss of regeneration potentiaLof the cells having T-DNA in their genomes.

2. Their large size makes the handling procedures during cloning tedious and cumbersome.

3. A general lack of unique cloning sites within the T-DNA, which are needed for the insertion of DNA segments to be cloned

In the above mention’s problems, 1 and 3 can removed by disarming and creating MCS, to overcome from 2nd problem need to develop

TLS Online TPP Program

#Question id: 19886

#Unit 13. Methods in Biology

Gene cloning refers to the

I) Production of large number of copies of the gene being cloned

II) Production of asexual progeny from a single individual or a cell 

III) Mechanism of intake of DNA fragments from the surrounding medium by a cell

TLS Online TPP Program

#Question id: 19887

#Unit 13. Methods in Biology

Paul Berg’s gene splicing experiment created the first rDNA molecules which was a

TLS Online TPP Program

#Question id: 19888

#Unit 13. Methods in Biology

There are some typical enzyme used in the manipulation of nucleic acid;

ENZYMES	USE IN NUCLEIC ACID MANIPULATION
A) klenow	i) DNA-dependent RNA polymerase
B) T4 DNA polymerase	ii) Single-strand specific nuclease
C) Taq DNA polymerase	iii) DNA pol I lacks 5’→ 3’ exonuclease activity
D) T7 RNA polymerase	iv) Thermostable DNA polymerase used in PCR
E) S1 nuclease	v) Lack  5’→ 3’ exonuclease activity

TLS Online TPP Program

#Question id: 19917

#Unit 13. Methods in Biology

Antigen-Antibody interactions are__

TLS Online TPP Program

#Question id: 19918

#Unit 13. Methods in Biology

Affinity constant (Ka) in between the antigen-antibody interaction can be analysed by which method?

TLS Online TPP Program

#Question id: 19919

#Unit 13. Methods in Biology

Scatchard plots are based on repeated equilibrium dialyses with a constant concentration of antibody and varying concentration of ligand. Which plots correctly verify the Scatchard equation that determined by equilibrium dialysis?