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#Question id: 18984


there are certain limitations of Recombinant Protein Production in E. coli in which presence of introns are one of them, to overcome from this

#Unit 13. Methods in Biology
  1. Oligonucleotide mutagenesis could be used to suitably modify the sequence of possible terminator sequences without altering the sequence of encoded amino acids.
  2. changing terminator sequences
  3. If such be the case, cDNA of the concerned gene must be used
  4. amino-acid composition