A pull down affinity chromatography assay is used for the investigation of

TLS Online TPP Program

#Question id: 18622

#Unit 13. Methods in Biology

Variants of PCR has been devised for mutational analysis

a) AS PCR

b) Site directed mutagenesis

c) Overlap extension PCR

d) Mega primer PCR

TLS Online TPP Program

#Question id: 18623

#Unit 13. Methods in Biology

Which of the following PCR has been devised to increase the specificity of PCR without lowering the efficiency

TLS Online TPP Program

#Question id: 18624

#Unit 13. Methods in Biology

In GFP protein oxidation of tripeptide sequence gives fluorescence the tripeptide sequence made up of

TLS Online TPP Program

#Question id: 18625

#Unit 13. Methods in Biology

m.RNA variation can be analyzed by using

TLS Online TPP Program

#Question id: 18644

#Unit 13. Methods in Biology

A laboratory technique used to separate DNA, RNA or protein molecules based on their size and electrical charge.

TLS Online TPP Program

#Question id: 18645

#Unit 13. Methods in Biology

SDS PAGE separate molecules by size because the presence of SDS (sodium dodecyl sulphate) denatures the protein removing 2˚, 3˚ and 4˚ structures (they assume a linear chain) and the SDS coats the molecules giving them a uniform charge/mass ratio. In an experiment (SDS-PAGE) with a discontinuous buffer system following band pattern is obtained 

   

Choose the correct statement regarding this band pattern