Practice Questions

TLS Online TPP Program

#Question id: 19181

Which of the following statement is incorrect regarding the cloning of PCR products?

#Unit 13. Methods in Biology

In cloning via restriction enzymes, restriction sites are induced before amplification is carried out
The restriction sites are induced in the primers before annealing
The intermediate molecules are having restriction sites at both ends
The amplified molecules can be cut at both the ends by appropriate enzymes

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TLS Online TPP Program

#Question id: 18815

#Unit 13. Methods in Biology

Which of the following PCR technique is best suited for detection of point mutation in DNA?

TLS Online TPP Program

#Question id: 18816

#Unit 13. Methods in Biology

The PCR technique used to preferentially amplify only one strand of the target DNA by using unequal primer concentration is

TLS Online TPP Program

#Question id: 18817

#Unit 13. Methods in Biology

The PCR technique widely used in bacterial genomic studies for screening of plasmid inserts is

TLS Online TPP Program

#Question id: 18818

#Unit 13. Methods in Biology

This PCR variant uses degenerate primers to amplify unknown sequences of DNA, related to a known DNA sequence is

TLS Online TPP Program

#Question id: 18819

#Unit 13. Methods in Biology

If the sequence of only one strand is known for primer synthesis, the best PCR variant that allows amplification of DNA with only one known sequence is

TLS Online TPP Program

#Question id: 18820

#Unit 13. Methods in Biology

The most efficient PCR technique that uses primer mix to detect deletions or duplications in a large gene is