This organism probably belongs to the same phylum as the 

TLS Online TPP Program

#Question id: 23175

#Unit 13. Methods in Biology

If we use a high-intensity light on to the fluorochrome there will initially be 

TLS Online TPP Program

#Question id: 23176

#Unit 13. Methods in Biology

When we use a high-intensity light to permanently bleach the fluorochrome in the patch, there will initially be no fluorescence and it will look 

TLS Online TPP Program

#Question id: 23177

#Unit 13. Methods in Biology

(FRET) technique uses a pair of fluorescent proteins in which the emission wavelength of the first is 

TLS Online TPP Program

#Question id: 23178

#Unit 13. Methods in Biology

If cyan fluorescent protein (CFP) and yellow  fluorescent protein (YFP) are present in close proximity  and emission light of wavelength 433nm is applied what will be observed.

TLS Online TPP Program

#Question id: 23179

#Unit 13. Methods in Biology

In order to observe FRET two flurophores must be in close proximity of about 10nm distance. Given below are few flurorescent molecules.

1. YFP

2. RFP

3. GFP

4. CFP

Which option below contains correct combination of flurorescent molecules that can give FRET?

TLS Online TPP Program

#Question id: 23180

#Unit 13. Methods in Biology

FRET is very sensitive to small changes in distance and in practice is not detectable at distances greater than