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TLS Online TPP Program
#Question id: 23245
#Unit 13. Methods in Biology
In Sanger chain termination method for sequencing requires?
1) A. Single stranded DNA template
2) Primer complementary to template strand
3) DNA Polymerase
4) dNTPs and ddNTPs
Which of the following option has correct combination?
TLS Online TPP Program
#Question id: 23246
#Unit 13. Methods in Biology
Which element in the Sanger sequencing actually terminates the chain elongation?
TLS Online TPP Program
#Question id: 23247
#Unit 13. Methods in Biology
In pyrosequencing which molecule is actually detected?
TLS Online TPP Program
#Question id: 23248
#Unit 13. Methods in Biology
Pyrosequencing is sequencing by synthesis whereby a PCR template is hybridised to an oligonucleotide and incubated with
1. DNA polymerase
2. ATP sulphurylase
3. Luciferase
4. Apyrase
Which of the following option has correct combination?
TLS Online TPP Program
#Question id: 23249
#Unit 13. Methods in Biology
Which enzyme converts the PPi to ATP in Pyrosequencing?
TLS Online TPP Program
#Question id: 23250
#Unit 13. Methods in Biology
In presence of ATP luciferase-mediated conversion of luciferin into which compound?