#Question id: 4496
#Unit 3. Fundamental Processes
Facilitates chromatin transcription was identified in human cell extracts, this factor makes transcription on chromatin templates much more efficient, choose correct statements;
#Question id: 4497
#Unit 3. Fundamental Processes
Elongation, termination of transcription, and RNA processing are interconnected, presumably to ensure their proper coordination,
|
Elongation factors |
|
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I. hSPT5 |
To recruit the 5’ -capping enzyme to the CTD tail of polymerase |
|
II. TAT-SF1 |
To recruits components of the splicing machinery to polymerase with a Ser-2 phosphorylated tail |
#Question id: 4498
#Unit 3. Fundamental Processes
Which enzyme is used in this particular mechanism;
#Question id: 4499
#Unit 3. Fundamental Processes
The final RNA processing event, polyadenylation of the 3’end of the mRNA, is intimately linked with the termination of transcription. Just as with capping and splicing, the polymerase CTD tail is involved in recruiting some of the enzymes necessary for polyadenylation,
#Question id: 4500
#Unit 3. Fundamental Processes
The free end of the second RNA is uncapped and thus can be distinguished from genuine transcripts. This new RNA is recognized by an RNase called, in yeast, _ and in humans_ that is loaded onto the end of the RNA by another protein that binds the CTD of RNA polymerase.
#Question id: 4501
#Unit 3. Fundamental Processes
According to Models of termination: torpedo and allosteric, which one is now the favored and why;
A. The Rat1 enzyme is very processive and quickly degrades the RNA in a 5’ -to-3’ direction, until it catches up to the still-transcribing polymerase from which the RNA is being spewed.
B. Termination depends on a conformational change in the elongating polymerase that reduces the processivity of the enzyme leading to spontaneous termination soon afterward.