TLS Online TPP Program
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TLS Online TPP Program
#Question id: 12296
#Unit 10. Ecological Principles
When calculating population growth rate, the symbol r refers to ________________.
TLS Online TPP Program
#Question id: 8906
#Unit 9. Diversity of Life Forms
What do hagfishes and lampreys have in common with the extinct conodonts?
TLS Online TPP Program
#Question id: 10511
#Unit 6. System Physiology – Plant
Which type of pumps inhibits digitoxin in the membranes of heart cells, leading to increased myocardial contraction?
TLS Online TPP Program
#Question id: 13088
#Unit 13. Methods in Biology
To express a yeast gene in E. coli, your task is to design a strategy to insert the yeast gene into the bacterial plasmid. Below is a map of the area of the yeast genome surrounding the gene in which you are interested.
The distance between each tick mark placed on the line above is 100 bases in length
Below are the enzymes you can use, with their specific cut sites shown 5’-XXXXXX-3’ 3’-XXXXXX-5’
The plasmid is 5,000 bases long and the two farthest restriction enzyme sites are 200 bases apart. The plasmid has an ampicillin resistance gene somewhere on the plasmid distal from the restriction cut sites.
You do the digestion of the insert and the vector and then ligate the two digestions together. You then transform the ligation into bacteria and select for ampicillin resistance. You get three colonies on your transformation plate. You isolate plasmid from each one and cut each plasmid with the enzyme XbaI. You then run your three digestions on an agarose gel and see the following patterns of bands. Describe what each plasmid actually was that was contained in each of the three colonies.
What is the Colony 1’s plasmid is;
TLS Online TPP Program
#Question id: 11328
#Unit 10. Ecological Principles
The __________ zone is the shallow area around the edge of a lake or pond with rooted vegetation, such as water lilies and pickerelweed.