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TLS Online TPP Program
#Question id: 12904
#Unit 13. Methods in Biology
Match the following:
1. Southern Blotting i. Melting temperature of DNA strand
2. DNA microchips ii.oligonucleotide matching to DNA sample
3. ASO-PCR iii.DNA hybridization to oligo arrays
4. DGGE iv. labeled probe hybridization to DNA
TLS Online TPP Program
#Question id: 12905
#Unit 13. Methods in Biology
Some gene/protein is used to identify the Disorder in humans
Match them correctly:
1. Tyrosine Kinase(TK) i. Atherosclerosis
2. HIV transactivator ii. Hypertension
3. Angiotensinogen iii. Kaposis Sarcoma
4. CET protein iv. Cardiac Hypertrophy
TLS Online TPP Program
#Question id: 12906
#Unit 13. Methods in Biology
An alternative method labeling strategy used in karyotyping and gene localisation is _______
TLS Online TPP Program
#Question id: 12907
#Unit 13. Methods in Biology
The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____
TLS Online TPP Program
#Question id: 12908
#Unit 13. Methods in Biology
Which statement is incorrect about CRISPR?
TLS Online TPP Program
#Question id: 12909
#Unit 13. Methods in Biology
According to the diagram, arrange the steps in correct order:
1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands.
2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows.
3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).
4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.