#Question id: 4465
#Unit 3. Fundamental Processes
Main function of Nus proteins in transcription,
#Question id: 4466
#Unit 3. Fundamental Processes
As well as synthesizing the transcript, RNA polymerase performs two proofreading functions on that growing transcript;
i. Pyrophosphorolytic editing
ii. Hydrolytic editing
Choose correct processes accordingly,
A. The polymerase backtracks by one or more nucleotides and cleaves the RNA product, removing the error-containing sequence.
B. In this, the enzyme uses its active site, in a simple back-reaction,
C. Catalyze the removal of an incorrectly inserted ribonucleotide, by reincorporation of PPi
D. The enzyme can then incorporate another ribonucleotide in its place in the growing RNA chain.
#Question id: 4467
#Unit 3. Fundamental Processes
Both polymerase removal and repair enzyme recruitment are performed by a single protein called?
#Question id: 4468
#Unit 3. Fundamental Processes
Major role of TRCF
#Question id: 4469
#Unit 3. Fundamental Processes
Termination is a normal and important function at the ends of genes. There, sequences called terminators trigger the elongating polymerase to dissociate from the DNA and release the RNA chain it has made, In bacteria, terminators come in two types: Rho-dependent and Rho-independent, choose correct statements about this;
Rho-dependent |
a- rut sites |
b- has an ATPase activity |
|
Rho-independent |
c- terminates only those transcripts still being transcribed beyond the end of a gene or operon |
d- intrinsic terminator |
#Question id: 4470
#Unit 3. Fundamental Processes
Most recent experiments suggest that the last of these is at least an important part of the story and that the conformational change causes the elongating complex to stall, with dissociation following more slowly- this conformational change caused by,