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TLS Online TPP Program

#Question id: 21250

A 56-year-old female was diagnosed with a tumor near the base of the skull, impinging on her optic tract. Which of the following statements about the central visual pathway is correct?

#Unit 7. System Physiology – Animal
  1. The fibers from each temporal hemiretina decussate in the optic chiasm, so that the fibers in the optic tracts are those from the temporal half of one retina and the nasal half of the other
  2. In the geniculate body, the fibers from the nasal half of one retina and the temporal half of the other synapse on the cells whose axons form the geniculocalcarine tract.
  3. Layers 2 and 3 of the visual cortex contain clusters of cells called globs that contain a high concentration of cytochrome oxidase.
  4. Complex cells have a preferred orientation of a linear stimulus and, compared to simple cells, are more dependent on the location of the stimulus within the visual field.
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TLS Online TPP Program

#Question id: 13075

#Unit 13. Methods in Biology

Radioisotopes must be used in RFLP and RAPD markers
TLS Online TPP Program

#Question id: 13076

#Unit 13. Methods in Biology

Restriction fragment length polymorphism denotes that a single restriction enzyme produces fragments of different lengths from the same stretch of genomic DNA of different strains of a species or from different related species. RFLPs are detected as follows:

i.  Large molecular weight genomic DNA is isolated from several strains or related species;

ii.  The fragments in these digests are separated through electrophoresis

iii.  These 'DNAs are then digested with a selected restriction enzyme

iv.  Exposed to a suitably radio-labelled appropriate DNA probe under conditions favouring DNA: DNA hybridization

v.  The resulting gel lanes are transferred and fixed to a suitable solid support and

vi.   The free probes are removed

vii.  The fragments to which the probe has hybridized are detected by filming them as distinct bands on a suitable photofilm through autoradiography.
TLS Online TPP Program

#Question id: 13077

#Unit 13. Methods in Biology

The DNA probes for RFLP may NOT be obtained from
TLS Online TPP Program

#Question id: 13078

#Unit 13. Methods in Biology

RFLPs have several unique advantages except this one;
TLS Online TPP Program

#Question id: 13079

#Unit 13. Methods in Biology

Name of that sophisticated equipment which used to the oligonucleotide, that serve as a primer in RAPDs is usually obtained by in vitro DNA synthesis,
TLS Online TPP Program

#Question id: 13080

#Unit 13. Methods in Biology

In RAPDs amplification will take place only of those regions of the genome that have the