TLS Online TPP Program

#Question id: 2217


In immunofluorescence studies you always see the small G-protein Ras in a specific region of the cell and, given its protein sequence along with many other proteins localized to this area, you hypothesize that their C-terminal Cys-Ala-Ala-X (X can be any amino acid) motifs may be responsible for targeting these proteins to this subcellular region. To test this hypothesis, you genetically engineer the sequence encoding this motif onto a cDNA encoding green fluorescent protein. When immunofluorescence is used, where specifically would you expect to see GFP localized in the cell?

#Unit 2. Cellular Organization
  1. in the nucleus

  2. in the cytoplasm

  3. in the Golgi

  4. at the plasma membrane