Following statements are regarding to dynamics of actin filaments.

A. When the concentration of G-actin is above the Cc, the filament end will grow; when it is less than the Cc, the filament will shrink.

B. ATP–G-actin is added much faster at the (+) end than at the (−) end, resulting in a lower critical concentration at the (+) end than at the (−) end.

C. At steady state, actin subunits treadmill through a filament. ATP-actin is added at the (+) end, ATP is then hydrolyzed to ADP and Pi, Pi is lost, and ADP-actin dissociates from the (−) end.

D. ATP–G-actin is added much faster at the (-) end than at the (+) end, resulting in a lower critical concentration at the (-) end than at the (+) end.

E. At steady state, actin subunits treadmill through a filament. ATP-actin is added at the (-) end, ATP is then hydrolyzed to ADP and Pi, Pi is lost, and ADP-actin dissociates from the (+) end.

Which of the following statements are correct?

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#Question id: 18654

#Unit 13. Methods in Biology

The pH of (i) stacking, (ii) resolving gel and (iii) tank buffer in SDS PAGE is respectively.

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#Question id: 18655

#Unit 13. Methods in Biology

In SDS PAGE sharpen bands are achieved by utilising differences in ionic strength and pH between the electrophoresis buffer (pH 8.8) and the stacking gel buffer (pH 6.8) and involves a phenomenon known as

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#Question id: 18656

#Unit 13. Methods in Biology

Which of the following is true about SDS-PAGE?

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#Question id: 18657

#Unit 13. Methods in Biology

In SDS PAGE band sharpening effect are observed, this band sharpening effect arises due to the

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#Question id: 18658

#Unit 13. Methods in Biology

when the separation of protein takes place based on their isoelectric point, the technique known as 

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#Question id: 18659

#Unit 13. Methods in Biology

Which of the following is true about for the fundamental of IEF