Which of the following steps is required to generate a knockout mouse?

a. microinjection of DNA into the pronucleus of a fertilized mouse egg

b. introduction of DNA into mouse embryonic stem cells

c. selection for cells containing the gene targeted insertion

d. expression of Cre protein during embryonic development

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#Question id: 18911

#Unit 13. Methods in Biology

All vectors used for propagation of DNA inserts in a suitable host are called………….. But when a vector is designed for the expression of, i.e., production of the protein specified by, the DNA insert, it is termed as ………..

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#Question id: 18912

#Unit 13. Methods in Biology

In PBR322,n in the AmpR there are restriction site are for PstI,  while tetracycline resistance have restriction site are for

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#Question id: 18913

#Unit 13. Methods in Biology

In PBR322, If a DNA with PstI will be selected on the basis of

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#Question id: 18914

#Unit 13. Methods in Biology

In PBR322, If a DNA with SalI and BamHI will be selected on the basis of

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#Question id: 18915

#Unit 13. Methods in Biology

In PBR322, A gene having terminal cut with the PstI, on which selectable marker the colony will be selected 

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#Question id: 18916

#Unit 13. Methods in Biology

In PBR322, A gene having terminal cut with the SalI and BamHI, on which selectable marker the transformed colony will be selected