TLS Online TPP Program

#Question id: 2863


The promoter of the araBAD operon from E. coli is activated in the presence of arabinose and the absence of glucose and directs expression of genes encoding enzymes required for arabinose metabolism. Unlike the cases of lac and gal genes, where a repressor and an activator work together, here two activators work together: AraC and CAP

+ arabinose

AraC binds that sugar and adopts a configuration that allows it to bind DNA as a dimer to the adjacent half-sites, araI1 and araI2

– arabinose

the AraC dimer adopts a different conformation and binds to araO2 and araI1, there is no monomer at site araI2, and so the protein cannot activate the araBAD promoter (araPBAD). This promoter is also controlled by CAP.

#Unit 2. Cellular Organization
  1. I-a, II-b

  2. II-b only

  3. I-a only

  4. I-b & II-a