The function and positioning of the histone variants can be studied by an application of technologies used in genomics. One useful technology is chromatin immunoprecipitation, or chromatin IP (ChIP). The correct sequence of the processes that occur in this experiment will be?

1. A histone variant with an epitope tag is introduced into a particular cell type, where it is incorporated into nucleosomes.

2. The pattern of hybridization on the microarray reveals the DNA sequences bound by the nucleosomes with the histone variant.

3. Antibody is added, and the nucleosomes containing the epitope-tagged histone variant are selectively precipitated.

4. Chromatin is isolated from the cells and digested briefly with micrococcal nuclease (MNase).

5. The DNA in these nucleosomes is extracted from the precipitate, labeled, and used to probe a microarray representing all or part of the genomic sequences of that particular cell type.

#Question id: 33484

#Unit 2. Cellular Organization

#Question id: 15703

#Unit 12. Applied Biology

#Question id: 33239

#Unit 1. Molecules and their Interaction Relevant to Biology

#Question id: 28463

#Unit 5. Developmental Biology

 First inducers of the lens are foregut endoderm and cardiac mesoderm, Foregut endoderm and cardiac mesoderm cause the synthesis of the factor in the head ectoderm during the late gastrula stage, these type of factor are____

#Question id: 1366

#Unit 4. Cell Communication and Cell Signaling

Mechano-transduction is the reciprocal interconversion of a mechanical force—or stimulus—and biochemical processes. These interconversions underlie a variety of biological activities, such as signaling, regulated gene expression, cell proliferation, cell migration, and interactions among cells and between cells and the ECM. Mechano-transduction in the context of cell-cell and cell-ECM interactions usually involves a cell-surface CAM or adhesion receptor that transmits mechanical force or biochemical information across the plasma membrane and one or more intracellular or extracellular mechano-sensors that respond to the mechanical stimulus by changing shape and activity. In Hypothetical model of the partial unfolding of a fibronectin type III, when that protein is subjected to mechanical force, following observations are-

A. Mechanical force generated within the cell by actin movement and transduce via multiple integrin adhesion receptors bound to the extracellular dimeric fibronectin can partially unfold the fibronectin.

B. The unfolding is thought to expose a putative, previously hidden binding site on fibronectin that has the potential to form β sheets with other fibronectin molecules, recruiting them to form fibronectin fibrils, and thus helping assemble the ECM.

According to observations which of the following protein act as mechano-sensor and mechano-transducer respectively?