The function and positioning of the histone variants can be studied by an application of technologies used in genomics. One useful technology is chromatin immunoprecipitation, or chromatin IP (ChIP). The correct sequence of the processes that occur in this experiment will be?

1. A histone variant with an epitope tag is introduced into a particular cell type, where it is incorporated into nucleosomes.

2. The pattern of hybridization on the microarray reveals the DNA sequences bound by the nucleosomes with the histone variant.

3. Antibody is added, and the nucleosomes containing the epitope-tagged histone variant are selectively precipitated.

4. Chromatin is isolated from the cells and digested briefly with micrococcal nuclease (MNase).

5. The DNA in these nucleosomes is extracted from the precipitate, labeled, and used to probe a microarray representing all or part of the genomic sequences of that particular cell type.

#Question id: 11277

#Unit 10. Ecological Principles

If a species is a keystone predator, then its removal from a community should

(A) Increase population size of the predator’s preferred prey

(B) decrease species diversity in the prey community

(C) decrease productivity of the predator’s preferred prey

(D) increase species diversity in the prey community

#Question id: 11278

#Unit 10. Ecological Principles

Which of the following is true with respect to oligotrophic lakes and eutrophic lakes?

A) Oligotrophic lakes are less subject to oxygen depletion.

B) Rates of photosynthesis are lower in eutrophic lakes.

C) Eutrophic lake water contains higher concentrations of nutrients.

D)Sediments in oligotrophic lakes contain larger amounts of decomposable organic matter.

#Question id: 11279

#Unit 10. Ecological Principles

Ecosystem A: Actual link 10, number of species 15  

Ecosystem B: Actual link 10, number of species 20   

Connectance of ecosystem A and B

#Question id: 11280

#Unit 10. Ecological Principles

Ecosystem services include processes that increase the quality of the abiotic environment. Which of the following processes would fall under this category?

#Question id: 11281

#Unit 10. Ecological Principles

Owls eat rats, mice, shrews, and small birds. Assume that, over a period of time, an owl consumes 5,000 J of animal material. The owl loses 2,300 J in feces and owl pellets and uses 2,500 J for cellular respiration. What is the production efficiency of this owl?

#Question id: 11282

#Unit 10. Ecological Principles

Which of the following ecosystems would likely have the largest net primary productivity per hectare and why?