#Question id: 532
#Unit 1. Molecules and their Interaction Relevant to Biology
In glycolysis, fructose 1,6-bisphosphate is converted to two products with a standard free-energy change (DG'°) of 23.8 kJ/mol. Under what conditions (encountered in a normal cell) will the free-energy change (DG) be negative, enabling the reaction to proceed to the right?
#Question id: 12110
#Unit 10. Ecological Principles
#Question id: 12161
#Unit 8. Inheritance Biology
#Question id: 4103
#Unit 3. Fundamental Processes
In a biochemical experiment, you compare the products from splicing reactions carried out in vitro using three different substrates. In each case the substrate is a construct containing a single intron surrounded by two exons, and in all cases the construct is the same overall size. But in one case, the intron is a group I intron, in another a group II intron, and in the third an intron removed by the spliceosome. Each construct is labelled in a manner that allows it to be detected after gel electrophoresis, and each is tested in two reactions—one, conditions that support self-splicing, and two, in the presence of nuclear extract as well. Note that, for simplification, only the final products of the splicing reaction are seen, but before degradation of the introns.
Which of the following observation is/are correct?
A. In lane A, the presence of nuclear abstract, a band for the lariat and the spliced product and pre-mRNA.
B. Lane B shows Group I Introns and Lane C is for Group II introns and reaction is nonself-splicing
C. Lane C is Group I intron. Splicing takes place in the absence and presence of nuclear abstract, and the reaction is self-splicing.
#Question id: 15366
#Unit 4. Cell Communication and Cell Signaling