Following statements are regarding to control of the G1–S phase transition in budding yeast.

A. G1 cyclin-CDK complexes stimulate the formation of G1/S phase cyclin-CDKs, which then initiate bud formation, centrosome duplication, and DNA replication. In yeast, the G1 cyclin gene is called CLN3.

B. Once sufficient Cln3 is synthesized from its mRNA, Cln3-CDK complexes phosphorylate and activate the transcriptional repressor Whi5.

C. Phosphorylation of Whi5 promotes its export out of the nucleus, allowing the transcription factor complex SBF to induce transcription of the G1/S phase cyclin genes CLN1 and CLN2 as well as other genes important for DNA replication.

D. Once produced, Cln1/2-CDKs contribute to further Whi5 phosphorylation. This positive feedback loop ensures the rapid accumulation of G1/S phase cyclin-CDKs.

Which of the following statements are correct?

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#Question id: 28164

#Unit 13. Methods in Biology

It is possible to calculate the number of clones that must be present in a gene library to give a probability of obtaining a particular DNA sequence. This correct formula is:

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#Question id: 28165

#Unit 13. Methods in Biology

Q. Arrange the steps of cloning and selection in YAC vector

a. BamHI digestion creates linear chromosome with telomeric ends.

b. Transformation

c. EcoRI digestion creates two arms, each with a selectable marker (X or Y).

d. Selection on the basis of marker

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#Question id: 28166

#Unit 13. Methods in Biology

The stability of YAC clones increases with the length of the cloned DNA segment, what is the minimum length require for stability as normal cellular chromosomes

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#Question id: 28167

#Unit 2. Cellular Organization

Organism and genes is given

Organism	Gene
a. yeast	I. 25,000
b. nematode worm	II. 6000
c. Arabidopsis	III. 21,700
d. 20,000 to 25,000	IV. 20,000 to 25,000

Match them correctly

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#Question id: 28168

#Unit 5. Developmental Biology

The cell has the capacity to differentiate into particular kinds of cells and achieve specific cell fates, this process is known as____

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#Question id: 28169

#Unit 5. Developmental Biology

At this stage the cell or tissue does not appear phenotypically different from its uncommitted state, its developmental fate has become restricted, process is known as___