The mitotic exit network that localizes to spindle pole bodies and whose activity is regulated by spindle position belongs to the family of signaling pathways known as the Hippo pathway in metazoans. Following statements are-

A. The conserved core of the Hippo signaling pathways consists of the Hippo protein kinase, which activates the Lats-Mob1 kinase.

B. The kinases are organized by a scaffolding molecule that, at least in budding and fission yeasts, targets the kinase cascade to centrosomes.

C. As in many kinase signaling cascades, a GTPase controls the activity of the mitotic exit network and the septation initiation network.

D. In metazoans, activation of the Hippo pathway leads to tissue overgrowth because the transcriptional activator TAZ (known as YAP in the mouse) constitutively promotes the expression of growth- and proliferation-promoting genes.

Which of the following statements are incorrect?

TLS Online TPP Program

#Question id: 12907

#Unit 13. Methods in Biology

The binding of a regulatory protein or transcription factor to a specific DNA site results in a complex which may be analyzed by the technique termed as _____

TLS Online TPP Program

#Question id: 12908

#Unit 13. Methods in Biology

Which statement is incorrect about CRISPR?

TLS Online TPP Program

#Question id: 12909

#Unit 13. Methods in Biology

According to the diagram, arrange the steps in correct order: 

1. DNA is then digested by the Cas9 nuclease at specific points on both DNA strands. 

2. Homologous recombination to repair the break and incorporate the new DNA sequence then follows. 

3.The nuclease Cas9 is targeted to DNA by a guide RNA consisting of a specific 20 nucleotide spacer/targeting sequence (crRNA).

 4. That identifies the DNA to be modified, linked to a trans-activating (tracrRNA) sequence necessary for the recruitment and stability of Cas9 nuclease.

TLS Online TPP Program

#Question id: 12910

#Unit 13. Methods in Biology

Match the following:

A. RNA analysis	i. Sexing or cell mutation sites	a. Expressed Sequence tag
B. Forensic science	ii. mRNA analysis	b. Analysis of DNA from blood
C. Gene discovery	iii. DNA profiling	c. Active latent viral infections
D. Single-cell analysis	iv. RT–PCR	d. Sex determination of unborn

TLS Online TPP Program

#Question id: 12911

#Unit 13. Methods in Biology

Match the following:

A. DNA polymerases	i. Alkaline phosphatase	a. RNA-dependent DNA polymerase, used in cDNA synthesis
B. Reverse transcriptase	ii. T4 DNA ligase	b. Thermostable DNA polymerase used in PCR
C. Ligases	iii. AMV-RT	c. Removes 5'-phosphates from DNA and RNA
D. Phosphatases	iv. Taq DNA pol	d. Links 5'-phosphate and 3'-hydroxyl ends via phosphodiester bond

TLS Online TPP Program

#Question id: 12912

#Unit 13. Methods in Biology

Name the enzyme which separates the double-helical DNA into single strands.