#Question id: 2969
#Unit 2. Cellular Organization
In the cells of many eukaryotic species, the nuclear envelope has to disappear to permit which of the following events in the cell cycle?
#Question id: 2970
#Unit 2. Cellular Organization
Describe how Cdc14 triggers the exit from mitosis in the budding yeast, S. cerevisiae.
#Question id: 2971
#Unit 2. Cellular Organization
Which one of the following is the most appropriate match?
|
Events |
Protein |
Phases |
|
A. Cohesins are loaded onto chromosomes |
I. Mei-S332/Shugoshin |
a. G1 |
|
B. Cohesins can dissociate from the DNA |
II. Polo kinase and Aurora B kinase |
b. G2 |
|
C. Prevents cohesin phosphorylation, and hence dissociation |
III. Scc2-Scc4 |
c. S |
|
D. Phosphorylation of cohesins |
IV. Pds5-Wapl |
d. M |
#Question id: 2972
#Unit 2. Cellular Organization
Match the following attachment (Column I) with definition(Column II).
|
Column I |
Column II |
|
A. Monotelic Attachment |
I. When sister kinetochores attach to microtubules emanating from opposite spindle poles, they are stably attached. |
|
B. Syntelic Attachment |
II. When one kinetochore attaches to microtubules emanating from two opposite spindle poles. |
|
C. Amphitelic Attachment |
III. When both sister kinetochores attach to microtubules emanating from the same spindle pole. |
|
D. Merotelic Attachment |
IV. When only one of the two sister kinetochores attaches to microtubules. |
Which of the following is correct?
#Question id: 2973
#Unit 2. Cellular Organization
Following statements are regarding to the spindle position checkpoint pathway in budding yeast.
A. During interphase and early mitosis, Cdc14 is sequestered and inactivated in the nucleolus. Inactive Tem1-GDP associates with the spindle pole body (SPB) nearest to the bud as soon as the mitotic spindle forms.
B. If chromosome segregation occurs properly, extension of the spindle microtubules inserts the daughter SPB into the bud, causing Tem1 to be activated by an unknown mechanism.
C. Tem1-GDP activates a protein kinase cascade, which then promotes the release of active Cdc14 from the nucleolus and exit from mitosis.
D. If the spindle apparatus fails to place the daughter SPB in the bud, Kin4 (cyan), an inhibitor of Tem1, is recruited from the parent cell cortex to the parent-cell-located SPB and maintains Tem1 in the GTP-bound form, and mitotic exit does not occur.
Which of the following are correct?
#Question id: 2974
#Unit 2. Cellular Organization
Following statements are regarding to control of the G1–S phase transition in budding yeast.
A. G1 cyclin-CDK complexes stimulate the formation of G1/S phase cyclin-CDKs, which then initiate bud formation, centrosome duplication, and DNA replication. In yeast, the G1 cyclin gene is called CLN3.
B. Once sufficient Cln3 is synthesized from its mRNA, Cln3-CDK complexes phosphorylate and activate the transcriptional repressor Whi5.
C. Phosphorylation of Whi5 promotes its export out of the nucleus, allowing the transcription factor complex SBF to induce transcription of the G1/S phase cyclin genes CLN1 and CLN2 as well as other genes important for DNA replication.
D. Once produced, Cln1/2-CDKs contribute to further Whi5 phosphorylation. This positive feedback loop ensures the rapid accumulation of G1/S phase cyclin-CDKs.
Which of the following statements are correct?