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TLS Online TPP Program
#Question id: 18661
#Unit 13. Methods in Biology
Which of the following highly sensitive analytical technique particularly useful for studying microheterogeneity in a protein
TLS Online TPP Program
#Question id: 18662
#Unit 13. Methods in Biology
Which of the following method is particularly useful for separating isoenzymes, which are different forms of the same enzyme, often differing by only one or two amino-acid residues.
TLS Online TPP Program
#Question id: 18663
#Unit 13. Methods in Biology
When a protein exists in mono-, di- and tri-phosphorylated forms. The difference of a couple of phosphate groups has no significant effect on the overall relative molecular mass of the protein, hence a single band on SDS gels, but the small charge difference introduced on each molecule can be detected by
TLS Online TPP Program
#Question id: 18664
#Unit 13. Methods in Biology
2D electrophoresis is the combination of
TLS Online TPP Program
#Question id: 18665
#Unit 13. Methods in Biology
In a road accident a forensic scientist collected traces of blood or other biological fluids and he wants to compared samples according to the composition of certain isoenzymes, for this which technique will efficiently work
TLS Online TPP Program
#Question id: 18666
#Unit 13. Methods in Biology
The above figure shows where sample applied was 100 µg of total protein extracted from a normal dog heart ventricle. The first dimension was conducted using a pH 4–7 isoelectric focussing gel. The second dimension was a 12% SDS–PAGE vertical slab gel. The pattern was visualised by silver staining. Which of the following techniques is applied