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TLS Online TPP Program

#Question id: 23631

cDNA libraries from liver cells are different than cDNA libraries from kidney cells because

#Unit 13. Methods in Biology
  1. the DNA is not the same in the two types of cells.
  2. reverse transcriptase acts differently in the two types of cells.
  3. mRNA is different in the two types of cells.
  4. polyA is present at 3สน ends of mRNA in one cell type only.
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TLS Online TPP Program

#Question id: 23628

#Unit 13. Methods in Biology

Which of the following is true concerning genomic libraries?
TLS Online TPP Program

#Question id: 23629

#Unit 13. Methods in Biology

Mechanical shearing is often the preferred method of fragmenting genomic DNA when constructing a genomic DNA library. Why would this method be preferred over complete digestion with restriction enzymes?
TLS Online TPP Program

#Question id: 23630

#Unit 13. Methods in Biology

The construction of a cDNA library is completed by
TLS Online TPP Program

#Question id: 23632

#Unit 13. Methods in Biology

Which formula may be implemented to determine the probability (P) that a library of a given size contains a particular clone? (N is the number of recombinant clones in the library; n is the frequency of occurrence of a desired clone)
TLS Online TPP Program

#Question id: 23653

#Unit 13. Methods in Biology

In a genomic DNA library the frequency of occurrence of a desired clone, n, also represents
TLS Online TPP Program

#Question id: 23654

#Unit 13. Methods in Biology

How many recombinant clones must be present in a cDNA library to be 95% certain that the library contains one clone of a particular mRNA molecule representing 0.005% of the total mRNA?