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TLS Online TPP Program

#Question id: 3763

What is the function of the enzyme topoisomerase in DNA replication?

#Unit 3. Fundamental Processes

  1. relieving strain in the DNA ahead of the replication fork caused by the untwisting of the double helix

  2. elongating new DNA at a replication fork by adding nucleotides to the existing chain

  3. reattaching the hydrogen bonds between the base pairs in the double helix

  4. building RNA primers using the parental DNA strand as a template

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TLS Online TPP Program

#Question id: 19843

#Unit 13. Methods in Biology

Real time PCR analyzed various steps such as__
a) Denaturation
b) Annealing
c) Phosphorylation
d) Amplification
e) Methylation
f) Extension
Which of the following steps are correctly analyzed by real time PCR?
TLS Online TPP Program

#Question id: 19844

#Unit 13. Methods in Biology

Match the following column, which correctly represent the qPCR done by three methods and measure such as;

                  COLUMN I

                    COLUMN II

A) SYBR green

i) Annealing

B) Taqman Assay

ii) Denaturation

C) Molecular beacon

iii) pH

 

iv) Amplification

 

v) Extension

TLS Online TPP Program

#Question id: 19845

#Unit 13. Methods in Biology

SYBR Green qPCR method is based on____I_______ with the _____II______ relationship.
TLS Online TPP Program

#Question id: 19846

#Unit 13. Methods in Biology

Variants of PCR has been developed on the basis of___
TLS Online TPP Program

#Question id: 19865

#Unit 13. Methods in Biology

Match the following activity of variants in Column I with their variants of PCR in Column II given below;

                     COLUMN I

                        COLUMN II

 

A) Enhance efficiency

 

i) Multiplex PCR

 

B) Sequence specific amplification

 

ii) Hot-start PCR

 

C) Mutational analysis

 

iii) Megaprimer PCR

 

 

iv) qPCR with SYBR

 

 

v) AS-PCR

 

 

vi) RLM-RACE

TLS Online TPP Program

#Question id: 19866

#Unit 13. Methods in Biology

Which of the following variants of PCR has been devised to increase the specificity of PCR without lowering the efficiency by variation annealing temperature?