which of the following best describes the complete sequence of steps occurring during every cycle of PCR?

1. The primers hybridize to the target DNA.

2. The mixture is heated to a high temperature to denature the double stranded target DNA.

3. Fresh DNA polymerase is added.

4. DNA polymerase extends the primers to make a copy of the target DNA.

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#Question id: 23161

#Unit 13. Methods in Biology

A classically used fluorescent dye which emits a orange light is?

TLS Online TPP Program

#Question id: 23162

#Unit 13. Methods in Biology

Match the fluorescent dyes in column I with emission light in column II

Column I                                                                          Column II

1. Texas                                                                            a. far red

2. Fluorescein                                                                   b. red

3. Rhodamine                                                                   c. blue 

4. Cy5                                                                               d. green

Which of the following option comprises all correct matches?

TLS Online TPP Program

#Question id: 23163

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in red light range?

TLS Online TPP Program

#Question id: 23164

#Unit 13. Methods in Biology

Out of the given below alexa dyes which one will emit in Blue light range?

TLS Online TPP Program

#Question id: 23165

#Unit 13. Methods in Biology

For visualizing two proteins which are localized in nucleus through fluorescence microscopy which two sets of dyes can be best used for clear visual?

TLS Online TPP Program

#Question id: 23166

#Unit 13. Methods in Biology

To visualize two proteins in fixed Hela cells using an epifluorescence microscope. Below is a list of various fluorophores (dyes) available to see those proteins.

1. FITC

2. GFP

3. Fluorescein

4. Alexa 568

Select the correct combinations of the appropriate dyes that would typically be utilized to observe co-localization in an epifluorescence microscope?