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TLS Online TPP Program

#Question id: 23849

Which pathway regulates the contraction of vertebrate smooth muscle? 

#Unit 2. Cellular Organization
  1. When the Ca2+ level rises → Ca2+ binds CaM→ MLC kinase becomes inactive → dephosphorylates the myosin LC → unfolds the myosin II→ assemble into bipolar filaments for contraction. 
  2. When the Ca2+ level rises→Ca2+ binds CaM→activates MLC kinase→ phosphorylates the myosin LC→unfolds the myosin II → assemble into bipolar filaments for contraction. 
  3. When the Ca2+  returns to its resting level → Ca2+ binds CaM → activates MLC kinase→ phosphorylates the myosin LC → unfolds the myosin II→ causing muscle contraction.
  4. When the Ca2+ level rises→ Ca2+ binds CaM→ activates MLC kinase → phosphorylates the myosin LC → myosin adopts a folded conformation→ assemble into bipolar filaments for contraction. 
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TLS Online TPP Program

#Question id: 18944

#Unit 13. Methods in Biology

A Linear or circular shuttle vector; behave in yeast like chromosomes; contain, ARS, CEN and telomeric sequences; used for cloning up to 1000 kb DNA is
TLS Online TPP Program

#Question id: 18945

#Unit 13. Methods in Biology

PCR can be used to generate single-stranded copies of a DNA sequence; this is called
TLS Online TPP Program

#Question id: 18946

#Unit 13. Methods in Biology

which of the following are Electrophoresis in free solution
TLS Online TPP Program

#Question id: 18947

#Unit 13. Methods in Biology

Separation of lipopolysaccharides and high molecular weight polysaccharides using thin layer
TLS Online TPP Program

#Question id: 18948

#Unit 13. Methods in Biology

The pore size of the gel depends on the which following two factors
TLS Online TPP Program

#Question id: 18949

#Unit 13. Methods in Biology

Rocket immunoelectrophoresis uses an agarose gel that contains uniform and definite concentration of th,e antibody. The buffer is so chosen that the antibody molecules have zero net charge. As the protein molecules migrate through the gel, they interact with the antibody molecules and 'rocket' -shaped regions containing immunoprecipitate are formed; which of the following is trure about Rocket immunoelectrophoresis